Staff Research Associate

Summary:The Research Associate will support the development and optimization of viral genome (VG) titer analytical methods within the Analytical Development group. The primary focus of this role is hands-on molecular biology work, including droplet digital PCR (ddPCR), qPCR, and related nucleic acid-based techniques. This individual will work closely with the team to develop robust, phase-appropriate assays for gene therapy programs and will receive scientific and technical direction from senior scientists. Duties/Responsibilities:Design, execute, and optimize VG titer assays using ddPCR (preferred) and/or qPCR platforms (e.g., QXOne, Bio-Rad)Perform primer and probe design and evaluate combinations for specificity, sensitivity, and reproducibilityConduct assay optimization studies including evaluation of cycling conditions, template input, and inhibitor effectsPerform nucleic acid purification (DNA and RNA) from a variety of sample types using standard extraction methodsApply restriction enzyme digestion and other molecular biology techniques as needed for assay developmentTroubleshoot assay failures and inconsistencies; document root cause investigations and corrective actionsGenerate, analyze, and interpret experimental data; prepare clear and accurate technical reports and lab recordMaintain compliant laboratory records in accordance with GMP/GLP documentation standardsCollaborate with cross-functional team members (QC, Bioinformatics, Regulatory CMC) as directedStay current with relevant scientific literature on ddPCR, NGS, and gene therapy analytics Qualifications/Requirements:Required:Hands-on experience with qPCR and/or ddPCR techniques, including assay setup, data analysis, and troubleshootingExperience with primer/probe design for PCR-based assaysProficiency in nucleic acid purification (DNA and RNA) and quantificationExperience with restriction enzyme applications and standard molecular cloning techniqueStrong attention to detail; ability to maintain accurate and complete laboratory recordsAbility to work effectively in a fast-paced, collaborative environment under scientific directionGood written and verbal communication skillsB.S. or M.S. in Molecular Biology, Biochemistry, Biotechnology, or a closely related life sciences fieldPreferred:Experience with droplet digital PCR (ddPCR) specifically (e.g., Bio-Rad QX200/QXOne)Familiarity with next-generation sequencing (NGS): library preparation, instrument operation, or data QCExperience working with AAV or other viral vectors in an analytical or research contextExperience in a GMP or GLP laboratory environmentFamiliarity with analytical method development and validation conceptsExperience with JMP or other statistical software for data analysis